Ekspresi Protein Gen E7 Human Papilloma Virus (HPV) Tipe 16 Secara SDS-Page



Suzana Devi, Yufri Aldy, Andani Eka Putra

Abstract


Human Papillomavirus (HPV) berkaitan dengan penyebab terjadinya kanker  serviks. Salah satu HPV yang beresiko tinggi adalah tipe 16. Protein virus yang beresiko tinggi yang menyebabkan karsinogen salah satunya gen E7. Dikarenakan tingginya tingkat kematian yang di sebabkan oleh kanker servik, salah satu cara untuk mengatasinya adalah dengan cara vaksinasi. Protein E7 merupakan target ideal untuk dapat dijadikan sebagai dasar dalam pembuatan vaksin. Dimana hasil ekspresi gen E7 HPV tipe 16 yang selanjutnya dapat dimanfaatkan dalam membuat antigen spesifik dari gen E7. Proses kloning gen E7 HPV tipe 16 dari peneliti sebelumnya, Isolasi plasmid menggunakan kit (Geneaid®), kultur bakteri yang telah ditumbuhkan pada plat yang mengandung antibiotik. Bakteri yang tumbuh merupakan bakteri yang telah mengandung gen sisipan. Selanjutnya lakukan over ekspresi. Hasil over ekspresi di tambah IPTG kemudian hasilnya di visualisasikan dengan SDS PAGE. Over ekspresi dari gen E7 HPV tipe 16 berhasil dilakukan dengan terdapatnya pita dari protein dari gen E7 HPV tipe 16 dari hasil SDS-PAGE. Dari penelitian yang telah dilakukan di dapat  hasil over ekspresi gen E7 HPV tipe 16 pada ukuran protein dengan kisaran 11,0 kDa. Pita yang didapat terlihat tipis dan samar-samar

Keywords


kanker servik; HPV tipe 16; ekspresi gen; SDS-PAGE

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