ISOLASI BAKTERI PATTOGEN PADA PASIEN PENDERITA INFEKSI TELINGA Chronic supparative otitis media (OMSK)

suryani suryani

Abstract


 

Abstrak

Tujuan penelitian ini adalah mengisolasi bakteri pattogen pada infeksi telinga Chronic suppurative otitis media yang nantinya sebagai tahap awal dari pengembangan analisa antimikroba Bakteri Asam Laktat (BAL) pada proses fermentasi santan kelapa menjadi Virgin Coconut  Oil (VCO), yang diharapkan  mampu  berfungsi sebagai antimikroba/antibakteri dari bakteri pattogen. Menurut Suryani dkk (2014), isolat BAL dari fermentasi santan mampu berfungsi sebagai antibakteri terhadap 5 bakteri uji  (E.coli NBRC14237, Staphylococcus aereus NBRC 13276, Bacillus substilis BTCCB, Salmonella thypii, dan Listeria  monocytogenes ). Untuk mendapatkan informasi tersebut maka di isolasi bakteri yang ada di cairan telinga pasien  penderita infeksi telinga Chronic suppurative otitis media dengan menggunakan  media umum  Blood Agar dengan metoda Pengenceran. Dari penelitian ini didapatkan 42 isolat dengan 4 jenis bakteri pattogen yaitu Pseudomonas aureginosa, Staphilococus aureus, Staphilococus epidermidis, Proteus mirabilis. Pasien OMSK yang diambil sekret nya 60 % berumur diatas 20 tahun dan 40% berumur dibawah 20 tahun yang sama banyak antara perempuan dan Laki-laki .

 

Kata Kunci:    Isolasi bakteri pattogen sekret pasien OMSK, Chronic suppurative otitis

media,BAL( Bakteri  Asam Laktat), Virgin Coconut Oil (VCO),

 

 

 

 

 

 

 

The Isolation of Pathogen Bacteria on The Sufferer of Ear Infection Chronic Supparative Otitis Media

1Suryani, 1Zulmardi,2 Abdi Dharma, 2Yunazar Manjang

1Universitas Muhammadiyah Sumatera Barat

2Universitas Andalas

E-mail [email protected]

 

Absract

This research is aimed to isolate pathogen bacteria in ear infection Chronic Supparative Otitis Media. This is the first phase in developing analysis Antimicroba Lactic Acid. Bacteria in the process of coconut milk fermentation into Virgin Coconut Oil (VCO). It is expected can be fanctioned as antibacteria of pathogen. According to Suryani et.al (2014), isolate of Antimicroba Lactic Acid of coconut milk fermentation can act as antibacteria against 5 examined bacteria (E.coli NBRC14237, Staphylococcus aereus NBRC 13276, Bacillus substilis BTCCB, Salmonella thypii, dan Listeria  monocytogenes). To achieve it, the ear liquid of sufferer containing bacteria is isolated with Blood Agar media and Dilution method. The result is that there 42 isolate in accordance with 3 kinds pathogen bacteria such as Pseudomonas aureginosa, Staphilococus aureus, Staphilococus epidermidis, Proteus mirabilis. The sufferer who has choosen as participant are 72% coming from the age of above 20 and the rest are under 20 years old. The ratio of sexes are similar between male and female.

Key Word:    Isolation pattogen bacteria sckret  OMSK, Chronic suppurative otitis

media, LAB(Lactat Acid Bacteria), Virgin Coconut Oil (VCO),

 

 


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References


Gopala Kresna.2010. Coconut Oil: Chemistry,Production and Its Applications –A ReviewIndian Coconut Journal. July 2010 .page 15-27

Handayani Rini, Joko Sulystio, Rita Dwi Rahayu. 2009. Extraction of Coconut Oil (Cocos nucifera L.) through Fermentation System. BIO DIVERSITAS Volume 10, Number 3, ISSN: 2085-4722 (electronic)Pages: 151-157

Luz-Adriana Sarmiento-Rubiano1, Bernard Berger2, Déborah Moine2, Manuel Zúñiga1, Gaspar Pérez-Martínez1,María J Yebra1. 2010. Characterization of a novel Lactobacillus species closely related to Lactobacillus johnsonii using a combination of molecular and comparative genomics methods. Sarmiento-Rubiano et al. BMC Genomics, 11:504

Mustaque Ali Mushtaque Ali Memon, Salman Matiullah (2008). Frequency of Un-Safe Chronic Suppurative Otitis Media in Patients with Discharging Ear JLUMHS MAY - AUGUST 2008.

Nguyen H.T.H., F.B. Elegado, N.T. Librojo-Basilio, R.C. Mabesaand E.I. Dizon. 2010. Isolation and characterisation of selected lactic acid bacteria for improved processing of Nemchua, a traditional fermented meat from Vietnam. Beneficial Microbes; 1(1): 67-74.

Prakash M, Laksmi K,Bacteriological Profile and Ttheir Antibiotic Susceptibility Pattern of Cases of Chronic Suppurative Otitis Media. Asian J Pharm Clin Res, Vol 6, Suppl 3, 2013, 210-212

Rajahmundry. 2012. Aerobic bacteriology of chronic suppurative otitis media in Rajahmundry, Andhra Pradesh, India. Biology and Medicine, 4 (2): 73-79.

Sadiah. 2011. Virgin coconut oil production by fermentation using Saccharomyces cerevisiae. Proceedings of the 2nd International Seminar on Chemistry 2011 (pp. 141-144)

Sri Redjeki and Ely Kurniati. 2013. The Kinetic Reacion of Virgin Coconut Oil (VCO) Fermentation in an Ideal Bioreactor Tank in a Batch Process. J. Chem. Chem. Eng. 7 : 159-163.

Shyamala and P Sreenivasulu Reddy. 2012. The study of bacteriological agents of chronic suppurative otitismedia - Aerobic culture and evaluation J. Microbiol. Biotech. Res., 2012, 2 (1):152-162

Suryani, Abdi Dharma. 2014. Antimicrobial and Antifungal Activity of Lactic Acid Bacteria Isolated from Coconut Milk Fermentation.Research Journal of Pharmaceutical, Biological and Chemical Sciences 5(6) page 1587-1595.

Suryani, Abdi Dharma. 2014. DDBJ (Data DNA Bank Jepang) (acession number)AB890143.




DOI: http://dx.doi.org/10.22216/jk.v1i2.1005

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